Autoclave Safety (Healthcare)

In the microbiology laboratory, the most widely used sterilizers are autoclaves. Autoclaves eradicate micro-organisms by use of pressurized steam to cause thermal stress. To achieve a sufficient kill rate, it is necessary to raise the temperature such that even the most thermo-tolerant micro-organisms are inactivated. This program covers the use, limitations and sterilization of autoclaves.

Topics covered in this safety video include: sterilization, limitations, temperature, time, contact, indicators, tape indicators, containment, autoclave safety, using the autoclave, and loading.

Course Outline

• Autoclave
• Limitations
• Time
• Contact
• Sterilization Indicators
• Autoclave Safety
• Loading
• Summary